New Host Record of Geminivirus on Bambara Groundnut (Vigna subterranea (L.) Verdc. (Fabaceae)) in East Java, Indonesia

Syaiful Khoiri, Eva Monica, Gita Pawana, Achmad Djunaedy, Dita Megasari, Tri Asmira Damayanti

Abstract


Bambara groundnut (Vigna subterranea L. Verdc.) is a legume plant originating from Africa. In Indonesia, Since 2006, the Bambara groundnut has been classified as a food crop based on the Decree of the Minister of Agriculture No:511/kpts/PD/310/9/2006. In East Java, bambara groundnut was planted in Gresik and Bangkalan regencies. In 2021, we recorded symptoms of virus infections, such as mosaic, stripes, dwarf, yellowing, and leaf malformations. However, information about the virus in Bambara groundnut (Vigna subterranea L. Verdc.) in Indonesia has not been reported. This study aims to detect the virus that infects the Bambara groundnut in East Java. This research was conducted using a survey method of Bambara groundnut in Gresik district and Bangkalan district. Certain symptoms such as mosaic, striped, stunted, yellow, and malformation were counted for the incidence of the disease. Samples of symptomatic plants were taken and brought to the laboratory for genomic extraction and analysis. DNA amplification was performed by RT-PCR using a pair of degenerative primers SPG1 and SPG2. The results of this study showed that the sample from the Gresik district was positively infected with the Geminivirus. Geminivirus infections were associated with yellowing and malformation of the leaves, and dwarf. This result record that Bambara is a new host of Geminivirus. But the species of the virus are not yet identified. The disease incidence of this disease caused by Geminivirus was 5.49%.

Keywords


Disease Incidence; Geminivirus; Molecular Detection; New Host; Yellow Virus; Viruses

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DOI: https://doi.org/10.21107/jsa.v1i1.4

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Journal of Science in Agrotechnology
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