Bioinsecticide Toxicity of Active Ingredients Serratia sp. with Bentonite Carrier Material on Spodoptera Litura and its Effect on Survival

Ahmad Zaiful Aziz, Gita Pawana, Sigit Prastowo

Abstract


Spodoptera litura is a polyphagus pest with a wide host range. In Indonesia, this pest is spread in almost all areas of soybean and horticultural crops. The attack level of S. litura on soybean plants varies greatly between, 23-45%. The case of S. litura resistance to synthetic insecticides encourages environmentally friendly control efforts using bioinsecticides with active ingredients from microorganisms. Serratia marcescens isolated from the soil is capable of producing prodigiosin, a chitinase enzyme, effectively killing pest insect and has the potential to be a biocontrol agent. This study aims to determined the toxicity of bioinsecticides with active ingedient  bacteria of which were suspected to be Serratia sp. with bentonite carrier material on S. litura and its impact on survival. Started with isolating Serratia sp bacteria from the rhizosphere taken from Bangkalan Regency with the D-E Shmit Ferguson climate category using media selective of CHROMagar™-Serratia. The isolates obtained were selected based on their ability to cause the highest mortality of level in the larvae of the beetle Tenebrio molitor. The isolate was cultured on nutrien agar medium added with 1.5% threhalose, then formulated in powder form with bentonite carrier. Toxicity as stomach  poison to S. litura was determined using probit analysis for LC50-90 and LT50-90 values. The results showed that at low concentration levels (0.01% and 0.1%) and short observation times (24 hours and 48 hours) the bioinsecticides were non-toxic, the test insects could survive to pupae but none of the pupae survived until adulthood.

Keywords


Bioinsecticide; Survival; Spodoptera litura; Serratia sp.; Toxicity

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References


Z. Afzal, U. Iqbal, A. Ghaffar, S. Hussain, Q. Adeel, H. Imam, and M. B. F. Hasmi, “Host Preference of Armyworm, Spodoptera litura under Laboratory Condition,” Acta Scientific Agriculture 7.3, pp. 45-48, 2023.

Yuliani and A. Utami, “Effectivity Test of Soursop Leaves (Annona Muricata) and Clove Leaves (Sizygium Aromatikum L.) Against Armyworm Control (Spodoptera litura),” Jurnal Pro-Stek, vol. 4, no. 1, 2022.

L. Prabaningrum dan T. K. Moekasan, “Ulat Gtayak Spodoptera spp.: Hama Polifag, Bioekologi dan Pengendaliannya,” IAARD PRESS Badan Penelitian dan Pengembangan Pertanian, 2022.

E. Uge, E. Yusnawan, Y. Baliadi, “Environmentally Friendly Control of Armyworms (Spodoptera litura Fabricius) on Soybean Uge,” Buletin Palawija vol. 19, no. 1, pp. 64-80, 2021.

W. T. A. Sanjaya, D. K. T. Sukmadewi, F. Hazra, A. Hasanah, D. A. Santosa, “Exploration of Potential Bacteria as Biological Control of Spodoptera litura,” J. Hort. vol. 30 no. 2, pp. 133-140, 2020.

S. R. Babu and B. Singh, “Resistance in Spodoptera litura (F.) to insecticides and detoxification enzymes,” Indian Journal of Entomology, pp. 90–94, 2023.

R. A. M. Ramadhan, N. S. Widayani, D. Dono, Y. Hidayat, and S. Ishmayana, “Resistance Level and Enzyme Activity of Spodoptera litura F. to Chlorpyrifos and Their Sensitivity to the Oil Formulation of Azadirachta indica Juss. and Cymbopogon nardus (L.) Rendl.,” AGRIVITA, Journal of Agricultural Science, vol. 44, no. 3, pp. 419–430, 2022.

F. Hernandez-Tenorio, A. M. Miranda, C. A. Rodríguez, C. Giraldo-Estrada, and A. A. Sáez, “Potential Strategies in the Biopesticide Formulations: A Bibliometric Analysis,” Agronomy, vol. 12, no. 11, p. 2665, 2022.

W. A. de Brito et al., “Botanical insecticide formulation with neem oil and D-limonene for coffee borer control,” Pesqui Agropecu Bras, vol. 56, 2021.

M. Homayoonzadeh, M. Ghamari, E. Torabi, K. Talebi, and J. Nozari, “A Novel Biopesticide Formulation for Organic Management of Aphis gossypii in Cucumber Greenhouses,” Chemistry Proceedings, vol. 10, no. 1, p. 55, 2022.

T. Clements, T. Ndlovu, and W. Khan, “Broad-spectrum antimicrobial activity of secondary metabolites produced by Serratia marcescens strains,” Microbiol Res, vol. 229, p. 126329, 2019.

A. Soenens and J. Imperial, “Biocontrol capabilities of the genus Serratia,” Phytochemistry Reviews, vol. 19, no. 3, pp. 577–587, 2020.

T. Clements, T. Ndlovu, S. Khan, and W. Khan, “Biosurfactants produced by Serratia species: Classification, biosynthesis, production and application,” Appl Microbiol Biotechnol, vol. 103, pp. 589–602, 2019.

K. L. Chin et al., “Application strategies by selective medium treated with entomopathogenic bacteria Serratia marcescens and Pseudomonas aeruginosa as potential biocontrol against Coptotermes curvignathus,” R. Soc. Open Sci., vol. 8, no. 4, p. 201311, 2021.

E. Konecka, J. Mokracka, S. Krzymińska, and A. Kaznowski, “Evaluation of The Pathogenic Potential of Insecticidal Strains to Humans,” Pol. J. Microbiol., vol. 68, no. 2, pp. 185–191, 2019.

F. Dwimartina, R. Rostaman, and L. Soesanto, “Keefektifan Bakteri Serratia Endosimbion Wbc Terhadap Ulat Grayak (Spodoptera Litura F.) Di Laboratorium Entomologi Bbpopt Jatisari Karawang,” Agro Wiralodra, vol. 3, no. 1, 2020, doi: 10.31943/agrowiralodra.v3i1.39.

J. Lee and D.-W. Lee, “Insecticidal serralysin of Serratia marcescens is detoxified in M3 midgut region of Riptortus pedestris,” Front Microbiol, vol. 13, 2022.

M. D. Lestari, N. Nukmal, E. Setyaningrum, S. Farisi, and A. Arifiyanto, “Larvicide Effects of Serratia marcescens strain MBC1 Extract on Instar III Larvae of Aedes aegyti,” Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH), vol. 9, no. 1, pp. 42–48, 2022.

E. C. Jensen, D. S. Katz Amburn, A. H. Schlegel, and K. W. Nickerson, “A collection of Serratia marcescens differing in their insect pathogenicity towards Manduca sexta larvae,” bioRxiv, pp. 2020–2027, 2020.

I M. Samudra, D. Susilowati, and Y. Suryadi, “The Application Of Biorational Pesticides On Paddy Stem Borer And Bacterial Leaf Blight,” Jurnal Penelitian Saintek, vol. 24, no. 1, 2019.

R. Jemi, S. Simanullang, G. Luhan, and D. N. Koroh, “Baeckea frutescen L. essential oil was tested with Aedes aegypti larvae at various concentration levels,” in IOP Conference Series: Materials Science and Engineering, p. 012002, 2021.

B. Pérez-Viso, S. Aracil-Gisbert, T. M. Coque, R. Del Campo, P. Ruiz-Garbajosa, and R. Cantón, “Evaluation of CHROMagarTM-Serratia agar, a new chromogenic medium for the detection and isolation of Serratia marcescens,” European Journal of Clinical Microbiology & Infectious Diseases, vol. 40, no. 12, pp. 2593–2596, 2021.

J. F. Campbell, C. G. Athanassiou, D. W. Hagstrum, and K. Y. Zhu, “Tribolium castaneum: a model insect for fundamental and applied research,” Annu. Rev. Entomol., vol. 67, pp. 347–365, 2022.

S. Devi, H. S. Saini, and S. Kaur, “Assessing the pathogenicity of gut bacteria associated with tobacco caterpillar Spodoptera litura (Fab.),” Sci. Rep., vol. 12, no. 1, p. 8257, 2022.

R. Fu, L. Zhou, K. Feng, X. Lu, J. Luo, and F. Tang, “Effects of Serratia marcescens (SM1) and its interaction with common biocontrol agents on the termite, Odontotermes formosanus (Shiraki),” J. For. Res. (Harbin), vol. 32, pp. 1263–1267, 2021.

X. Xia, W. Wu, J. Chen, and H. Shan, “The gut bacterium Serratia marcescens mediates detoxification of organophosphate pesticide in Riptortus pedestris by microbial degradation,” Journal of Applied Entomology, vol. 147, no. 6, pp. 406-415 2023.

K. Heu et al., “The effect of secondary metabolites produced by Serratia marcescens on Aedes aegypti and its microbiota,” Front Microbiol, vol. 12, p. 645701, 2021.

C. Aggarwal, S. Paul, V. Nain, V. Tripathi, B. Paul, and M. A. Khan, “Comparative response of Spodoptera litura challenged per os with Serratia marcescens strains differing in virulence,” J Invertebr Pathol, vol. 183, p. 107562, 2021.

M. Nadziakiewicza, S. Kehoe, and P. Micek, “Physico-chemical properties of clay minerals and their use as a health promoting feed additive,” Animals, vol. 9, no. 10, p. 714, 2019.

D. B. Tagiev, O. Y. Golubeva, S. A. Mamedova, U. A. Mamedova, N. A. Alikina, E. Y. Brazovskaya,” Comparative Study of the Physical and Chemical Properties of Natural Bentonite Clay of the Dash-Salakhli Deposit and Synthetic Montmorillonite,” Glass Physics and Chemistry, vol. 48, no. 2, pp. 140–150, 2022.

G. Sutio, A. N. U. R. Afifah, R. Maharani, and M. Basri, “Serratia marcescens strain NPKC3_2_21 as endophytic phosphate solubilizing bacteria and entomopathogen: Promising combination approach as rice biofertilizer and biopesticide,” Biodiversitas, vol. 24, no. 2, 2023.

N. Jupatanakul, J. Pengon, S. Selisana, W. Choksawangkaran, N. Jaito, A. Seung, R. Bunyong, N. Posayapisit, K. Tammatinna, N. Kalponungkul,“ Serratia marcescens secretes proteases and chitinases with larvicidal activity against Anopheles dirus,” Acta Trop, vol. 212, p. 105686, 2020.

S. A. Martínez-Zavala, U. E. Barboza-Pérez, G. Hernández-Guzmán, D. K. Bideshi, and J. E. Barboza-Corona, “Chitinases of Bacillus thuringiensis: Phylogeny, modular structure, and applied potentials,” Front Microbiol, vol. 10, p. 3032, 2020.




DOI: https://doi.org/10.21107/jsa.v2i1.13

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Journal of Science in Agrotechnology
ISSN: 2986-1411
Published by: Universitas Trunojoyo Madura, Indonesia
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